General chamber preparation for
semen testing
Mix the sample thoroughly just before testing.
Pipette one
drop
(approximately 4 microliters) of specimen. Place the specimen at
the extreme edge of one of the
sampling areas (figure A). Two tests
can be performed using one Cell-VUŽ slide.
Make
sure the Cell-VUŽ name on the cover glass is facing the observer
as the grid is etched on the reverse
side.
Gently lower
the
cover glass over the specimen so that the edge of the cover glass
just covers the sample (figures B
and C).
Slide the cover glass into position as shown in figure D. This will
eliminate
air bubbles from the counting area.
Using Cell-VUŽ for sperm counts and
motility
Isolate and view the CELL-VUŽ grid. This grid is divided into 100
small boxes each 0.1 x 0.1 mm.
For
undiluted
sperm count all motile and non-motile sperm within 10 small boxes of
this grid.
Divide this number by 2. This result is the concentration
of sperm in millions/ml.
|
% Motility = |
# Motile
Sperm
|
x 100 |
|
|
# Motile
Sperm
+
# non-
Motile Sperm
|
Suggestions:
For increased
accuracy, count all sperm within the entire grid (100 boxes). Multiply
the count by 50,000 to obtain the total concentration of sperm/ml.
Sperm can be
immobilized by placing a small amount of sample in an
appropriate
container and then immersing it in hot water for several minutes
If diluted
specimen is used, follow the
instructions and multiply by the dilution
factor. |
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